
The egg white, or albumen, of a chicken egg is composed of four layers: the chalaziferous layer, the inner thin layer, the viscous thick layer, and the outer thin layer. The layers account for about 2.7%, 17%, 57%, and 23% of the total volume of the egg white, respectively. The chalaziferous layer is a dense albumen that contains numerous mucin-like fibers and forms a thin, compact structure around the egg yolk, keeping it in the center of the egg. The inner and outer thin layers contain a higher proportion of water-soluble proteins, and these layers increase when eggs are stored. The thick layer is added first and is closest to the yolk, providing the majority of riboflavin and protein for the embryo.
| Characteristics | Values |
|---|---|
| Number of layers | 4 |
| Layer composition | Thick and thin layers |
| Layer order | Thick, inner thin, viscous thick, outer thin |
| Layer functions | Stabilizing yolk movement, protecting the yolk, providing nutrition for the embryo |
| Layer percentages of total volume | 2.7%, 17%, 57%, 23% |
| Thick layer function | Provides majority of riboflavin and protein for the embryo |
| Thin layer function | Protecting the yolk |
| Thick layer composition | Dense albumen with embedded mucin-like fibres |
| Thin layer composition | Higher proportion of water-soluble proteins |
| Other components | Ovalbumin, conalbumin/ovotransferrin, ovomucin, lysozyme, ovomucoid |
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What You'll Learn

The four layers of chicken albumen
The chicken albumen, commonly known as the egg white, is a clear liquid formed from the layers of secretions of the anterior section of the hen's oviduct. It is composed of four layers, each with its own unique function, and accounts for about two-thirds of a chicken egg by weight.
The first layer, known as the chalaziferous layer, is closely applied around the yolk membrane. This layer contains dense albumen with numerous mucin-like fibers, forming a thin and compact structure that helps to stabilize the yolk, keeping it in the center of the egg. The chalaziferous layer also contains a high amount of ovomucin, a glycoprotein that contributes to the gel-like structure of thick albumen.
The second layer is the inner thin layer, which acts as a protective capsule enveloping the yolk. This layer contains a higher proportion of water-soluble proteins, and its volume increases when eggs are stored. The inner and outer thin layers work together to provide a strong defense against bacterial invasion, with the outer thin layer also serving as a protective barrier against pathogens.
The third layer is a viscous thick layer that provides the majority of the riboflavin and protein for the developing embryo. This layer is added first and is closest to the yolk, supplying essential nutrients for the growth of the embryo. The thick layer also contains rope-like structures called chalazae, which help to hold the yolk in place as it moves through the reproductive tract.
The fourth and outermost layer is the outer thin layer, which also serves as a protective barrier. This layer is primarily composed of water-soluble proteins and plays a crucial role in safeguarding the yolk from microbial infections. The outer thin layer is the first line of defense against pathogens, ensuring the embryo's safety and overall health.
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The function of each layer
A chicken albumen, or egg white, is composed of four alternating layers of thick and thin albumen. Each layer contains different substances and serves a specific function.
The inner thick layer, also known as the chalaziferous layer, is dense and contains numerous mucin-like fibres. This layer forms a compact structure around the egg yolk, stabilising it and keeping it in the centre of the egg. It also contains high levels of lysozyme, a holoprotein that can lyse the wall of certain Gram-positive bacteria.
The inner thin layer acts as a protective capsule, enveloping the yolk. It contains a higher proportion of water-soluble proteins, which increase when the eggs are stored. This layer also includes ovalbumin, the most abundant protein in albumen. Ovalbumin is a heat-resistant phosphoglycoprotein that can withstand temperatures up to 84°C.
The outer thin layer, or thin albumen, also contributes to the structural stability of the egg. It contains a higher proportion of water-soluble proteins compared to the thick layer. Additionally, this layer is a source of ovomucin, a glycoprotein that contributes to the gel-like structure of the thick albumen. The amount of ovomucin in the thin albumen is four times less than in the thick albumen.
The outermost thick layer, or thick albumen, is responsible for providing additional protection and support to the inner layers. It contains a higher concentration of ovomucin, which maintains the viscosity and structure of the egg white. Ovomucin also helps prevent bacterial invasion by blocking the entry of pathogens.
Overall, the layers of the chicken albumen work together to stabilise the yolk, provide essential nutrients for the growth of the embryo, and protect the egg's structural integrity.
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The composition of each layer
The albumen, or egg white, of a chicken egg is composed of four layers, each with a unique function and composition. Firstly, the chalaziferous layer, also known as the inner thick layer, is formed closely around the yolk membrane. This layer contains dense albumen with embedded mucin-like fibres, creating a thin and compact structure that stabilises the yolk, keeping it centred in the egg. The chalaziferous layer also contains glycoproteins such as ovomucin, which contribute to the gel-like structure of thick albumen.
The second layer, the inner thin layer, acts as a protective capsule enveloping the yolk. This layer, along with the outer thin layer, contains a higher proportion of water-soluble proteins. These layers increase in volume when eggs are stored, and their primary function is to protect the yolk and provide defence against bacterial invasion. The thin layers are made partly of keratin, a protein also found in human hair, and their strength can be surprising when pulled or tugged.
The third layer is the viscous thick layer, which provides the majority of riboflavin and protein for the developing embryo. This layer is added first and is closest to the yolk. The thick layer also contains rope-like structures called chalazae, which appear on both sides of the yolk and hold it in place as it moves through the hen's reproductive tract. The chalazae are rich in lysozyme, a holoprotein that can lyse the wall of certain Gram-positive bacteria, providing additional protection to the embryo.
The fourth and outermost layer is the outer thin layer, which, along with the inner thin layer, accounts for a significant proportion of the total volume of the egg white. This layer also contributes to the overall protection and structural integrity of the egg, ensuring the yolk remains centred and secure. The outer thin layer is essential in maintaining the quality and freshness of the egg, as it helps prevent bacterial growth and maintains the egg's shape and consistency.
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How the layers are formed
A chicken egg is composed of various layers, each serving a specific function. The process of egg formation is intricate and remarkable, showcasing the wonders of nature. The layers are formed through the secretion of the anterior section of the hen's oviduct during the egg's journey through the oviduct.
The first layer to form is the albumen, also known as the egg white, which accounts for around two-thirds of the egg's weight. The albumen is primarily composed of about 90% water and 10% proteins, including albumins, mucoproteins, and globulins. This layer forms around the fertilized or unfertilized egg yolk, acting as a protective barrier and providing additional nutrition for the embryo's growth if the egg is fertilized. The albumen itself consists of four alternating layers of thick and thin albumen, with the thick albumen having a gel-like structure due to the presence of ovomucin, a type of glycoprotein. The inner thin layer of the albumen lies close to the chalazae, serving as a protective capsule for the yolk.
Following the formation of the albumen, the shell membrane develops. This thin layer, composed of two transparent protein membranes made partly of keratin, takes about 1.25 hours to form and acts as a defence against bacterial invasion. The outer membrane adheres to the eggshell, while the inner membrane sticks to the albumen.
The next layer is the eggshell, which forms in the hen's uterus, also known as the shell gland. This process takes approximately 19 hours, during which pigments are added to the shell. Initially, all eggshells are white, but depending on the breed of chicken, they can turn blue or brown due to the addition of pigments.
Lastly, the cuticle or bloom is the outer coating of the shell, which helps reduce moisture loss and further prevents bacterial penetration. This layer is mostly removed from table eggs during mechanical washing.
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The importance of albumen quality
Albumen, or egg white, is a crucial component of chicken eggs, providing essential protection and nutrition to the developing embryo. Its quality is of utmost importance to both consumers and producers, impacting the overall health of the embryo and the nutritional value of the egg.
The albumen is composed of four layers, each with a specific function. The innermost layer, known as the chalaziferous layer, surrounds the yolk, stabilising it and maintaining its central position. This layer is crucial in ensuring the embryo's safety and proper development. The second layer, the inner thin layer, acts as a protective capsule, providing an additional barrier against potential harm. The third layer, the thick albumen, is responsible for the albumen's viscoelasticity and overall durability. Finally, the outer thin layer contributes to the overall structural integrity of the egg.
The quality of the albumen is influenced by various factors, including the hen's diet, magnum morphology, rate of albumen secretion, storage time, and temperature. A diet rich in natural products of plant and animal origin has been found to improve albumen quality. For example, isoleucine in a low CP diet enhances albumen quality, while threonine improves egg quality in birds with low CP levels by increasing the secretion of digestive enzymes and beneficial gut microflora. Additionally, adequate essential amino acids (AA) are critical in maintaining the structural integrity of the egg and enhancing its external and internal quality.
Maintaining high-quality albumen is essential for several reasons. Firstly, it ensures the proper development and health of the embryo. High-quality albumen provides the necessary nutrients, including proteins, vitamins, minerals, and amino acids, for the embryo's growth. Secondly, it contributes to the nutritional value of the egg for human consumption. The albumen is a rich source of high-quality protein, containing all nine essential amino acids, making it a complete protein source. Thirdly, high-quality albumen improves the functional properties of the egg, such as its ability to create a foam when beaten, which is essential for various culinary applications. Lastly, high-quality albumen ensures better protection against bacterial and microbial infections, preserving the embryo's health and the egg's overall freshness.
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Frequently asked questions
There are four layers in a chicken albumen, also known as egg white.
The four layers of a chicken albumen are the chalaziferous layer, the inner thin layer, the viscous thick layer, and the outer thin layer.
The chalaziferous layer is the inner thick layer resting around the yolk, which stabilizes the yolk movement and maintains its centre position.
The inner and outer thin layers contain a higher proportion of water-soluble proteins, and these layers increase when eggs are stored.











































