
Determining whether a chick embryo is dead is a delicate process. One common method is candling, which involves holding the egg to a light source to observe development. However, this method can be challenging as early embryo deaths are difficult to detect, and excessive handling can damage the embryo. Other signs of embryo death include the presence of clear fluid and a dark ring surrounding the embryo. In some cases, embryo mortality may be attributed to deficiencies or external factors such as ventilation problems. While it's mainly up to chance, ensuring healthy parents and a safe nest during incubation can improve the odds of viable chicks.
| Characteristics | Values |
|---|---|
| Embryo Mortality | Hatcher mortality is embryo mortality occurring after day 18. |
| Cause of Embryo Mortality | Factors include age, nutrition, health status of the breeder flock, egg handling, incubation, and long-lasting problems. |
| Candling | Holding an egg to a light source to determine if it is still fertile. |
| Viable Embryo Characteristics | Dark spot with veins spidering out from it and movement within seconds of candling. |
| Dead Embryo Characteristics | Clear space under the air cell, blotchy and lumpy shadow, no movement in later stages, embryo surrounded by a dark ring. |
| Float Test | A test to determine if an egg is still viable by placing it in water. |
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What You'll Learn

Candling the egg
Candling is a process that involves shining a bright light through an egg to illuminate its contents and check on the development of the embryo. It is a good way to determine the fertility rate of an egg and can be performed as early as day 4 of incubation. It is best to candle eggs in a dark room, and eggs can be out of the incubator for up to 30 minutes, but no longer.
On day 4, you should be able to see blood pumping through the heart of a tiny, developing embryo. If the embryo has died at this point, you may still see a faint network of blood vessels inside the egg. From days 6-8, a dead embryo will show a large, black eye. By day 10, the chick will be fully formed, with small down feathers. If the down is matted and sticky, this could be due to a turning issue during days 4-8.
From days 11-14, a chick will be small, fully formed, with down and toenails. It is unusual for embryos to die during this period, but if they do, it is likely due to bacterial or mould contamination. By day 18, the chick's body is filling most of the egg.
Some sources suggest that candling eggs at 18 days is a good idea, but others argue that after 14 days, there is not much to see other than the increasing air sac size. It is important not to candle eggs after day 17 as any movement can harm the chick.
When candling, it is important to avoid bumping or jostling the egg, especially during the early days, as the delicate blood vessels are still developing, and disturbing them could harm the embryo. It is also important to ensure your hands are clean and dry, as oil from your fingers can clog the pores in the egg shell and prevent the embryo from getting oxygen.
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Embryo development knowledge
Candling:
Candling is a crucial technique used to assess embryo development. It involves holding an egg to a light source, allowing you to see inside the shell and evaluate the embryo's growth. This method can help identify fertile eggs, detect cracks in the shell, and determine the viability of the embryo. It is recommended to start candling after the first few days of incubation to avoid damaging the fragile embryo.
Signs of a Healthy Embryo:
During candling, a viable embryo will present specific characteristics. It will appear as a dark spot with veins radiating from it, indicating the development of blood vessels. Additionally, movement within seconds of candling is a positive sign, signifying that the embryo is alive and progressing normally.
Embryo Mortality:
Embryo mortality can occur at different stages of development, and it is essential to identify the potential causes to reduce future losses. One of the leading causes of embryo death is deficiencies in the early stages of development, which can be challenging to detect. Other factors include the age, nutrition, and health status of the breeder flock, as well as the quality of egg handling and incubation conditions.
Hatcher Mortality:
Hatcher mortality refers to embryo deaths occurring after day 18 of incubation. These deaths are usually attributed to long-lasting problems rather than immediate conditions. Analyzing hatch debris and categorizing dead embryos by developmental stage can provide insights into the causes of hatcher mortality.
Malpositions:
Improper positioning of eggs during incubation can lead to reduced hatch rates. One common issue is the "head over wing" position, which suggests overheating during the final days of incubation. Another sign to look out for is the presence of dead chicks in multiple hatcher baskets, which could indicate ventilation problems in the hatcher.
Staggered Hatching:
Sometimes, chick embryos may hatch at different times, which can be confusing for those expecting a synchronized hatch. It is important to remain patient and continue providing the necessary care and attention to the eggs, as some may simply be developing at a different pace.
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Float test
The float test is a method to determine whether a chick embryo is alive or dead. It is important to note that the test can be mildly traumatic for a chick inside an unpipped egg, so it should only be performed when you are ready to dispose of the unhatched eggs.
To perform the test, fill a container with water that is roughly baby bottle temperature, around 100 degrees Fahrenheit. You can test the temperature by touching the water to the underside of your wrist – it should feel barely warm.
Next, carefully place the egg in the water and observe its position. There are four possible outcomes:
- Sinker: If the egg sinks, it is likely that the embryo never developed, and the egg is a dud.
- High floater: If the egg floats high in the water with 45% or more of its surface above the waterline, it indicates that the internal membranes have ruptured, causing the egg to dehydrate. This suggests that the embryo has died.
- Low floater: If the egg floats low in the water, it indicates that the embryo is viable and has developed full-term.
- Low floater with movement: If the egg floats low and is rocking and rolling in the water, it is a definite sign that the embryo is alive and moving inside the egg.
It is important to note that the float test does not indicate whether an egg is safe to eat or not. While it can provide an approximate age of the egg, a freshly laid egg may harbour salmonella and sink in the test, while a floating egg may be quite old but still safe to consume.
Additionally, it is crucial to closely inspect the eggs before performing the float test. If the shell is broken, pipped, or cracked, water can enter and cause the chick to drown.
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Listen for tapping
When it comes to determining whether a chick embryo is alive or dead, listening for tapping sounds inside the egg is a crucial method. This technique is non-invasive and provides valuable insights into the embryo's development without causing any harm or disturbance. Here are some detailed instructions on how to listen for tapping to ascertain the status of a chick embryo:
Timing is Key
It is important to listen at the right time to avoid false results. For chicken eggs, start listening after 10 days for a typical 21-day incubation period. For duck eggs, the listening period is from day 20 to 23 during their 28-day incubation. Waiting until the final third of the incubation period is essential because the embryo needs to be developed enough to make audible noises.
Sanitize and Prepare
Before handling the eggs, always remember to sanitize your listening tools and wash your hands thoroughly. This step is crucial to avoid transferring bacteria and potentially harming the embryo.
Get Close and Listen Carefully
Hold the large end of the egg up to your ear and listen intently for any faint tapping or scratching sounds. These subtle noises indicate the embryo's movements inside the egg and are positive signs of life. The embryo might be tapping in an attempt to hatch and break free from the egg.
Encourage a Response
If you don't hear any sounds initially, try lightly tapping on the egg yourself. A live embryo may respond to this stimulus by tapping back. You can also try lightly scratching the egg with your fingernail to encourage movement.
Combine with Other Methods
Listening for tapping can be combined with other techniques such as candling, floating, and weighing. Candling involves shining a bright light through the egg to observe embryo development and identify any issues early on. Floating an egg in water can also indicate its freshness and viability. Weighing eggs periodically helps determine if the embryo is progressing normally, as sudden weight changes could signal problems.
Signs of a Dead Embryo
If there are no tapping sounds towards the end of the expected incubation period, the embryo is likely dead. Other signs include the egg being cold to the touch, a cracked eggshell, an unpleasant odor, or the presence of clear fluid inside the egg.
By following these steps and listening carefully for tapping sounds, you can effectively determine whether a chick embryo is alive or dead, ensuring the proper care and management of the hatching process.
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Ventilation problems
Embryos that die due to insufficient ventilation are usually fully developed and can die late, after day 18. Ventilation becomes increasingly critical after day 15. The absorption of the intestines and the yolk sac can help identify when the embryo died. Absorption of the intestines begins on day 15, followed by absorption of the yolk, which begins on day 18 and continues through day 19 until internal pipping. This process requires an unlimited availability of oxygen, physical power, and space inside the body cavity. If the embryo is weak and exhausted, it may not be able to complete the process and close the navel.
Late embryo mortality is complex and can be caused by insufficient or non-uniform ventilation during the final days of incubation. Large temperature differences within a setter may be due to excessive humidifier, cooler, and heater activity. This can be caused by technically challenging set points for the incubator or incorrect physical air supply parameters, imbalanced supply and exhaust pressures, or inefficient internal air mixing.
To prevent ventilation problems, ensure that the brooder is ventilated but draft-free. Air should be able to circulate without blowing on the chicks and causing them to chill. The temperature should be correct for the age of the chicks and stable. If all the chicks are huddled together near the heat source, they are too cold. If they are all trying to stay away from the heat source, the brooder is too warm.
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Frequently asked questions
Candling is a process where you hold the egg up to a light source to determine whether the egg is still fertile. If the embryo is dead, you will see a clear space develop under the air cell and the shadow of the chick will look blotchy and lumpy.
You can purchase a candling device or make your own with a few materials. In a dark room, hold the large end of the egg up to the light. The light will shine on the egg, allowing you to see what's happening inside the shell.
In the later stages of development, embryo death is harder to detect, but there will be no movement from the embryo.
If the chick is dead, there will be cloudy fluid on top of the chick (under the membrane). If you crack the egg, it will be yellow.

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