
The reproductive system of a chicken is fascinating, and understanding it is essential for anyone looking to raise chickens for eggs. In this system, the male and female reproductive organs differ significantly. In male chickens, the testes produce sperm, which is transported through the vas deferens, while female chickens have only one mature gonad (the ovary) and a series of tubes in the oviduct where sperm can be stored for later fertilization. This storage area is known as the sperm nest, and its presence allows hens to maximize fertilization without the need to mate again for each egg. This paragraph will explore the location of this sperm nest in female chickens and its role in their reproductive system.
| Characteristics | Values |
|---|---|
| Location of sperm nest | In the oviduct of hens |
| Alternative names | Sperm storage tubules, pouches, nests |
| Number | 2000-3000 |
| Location | Either in the lower portion of the infundibulum or the upper portion of the vagina at the utero-vaginal junction (UVJ) |
| Function | To collect and store semen for later fertilization of eggs |
| Fertilization period | 1 week of high fertility, 2 weeks of some fertility, 3 weeks of low fertility, 4 weeks of little to none |
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What You'll Learn

Sperm is stored in the oviduct of hens
The reproductive system of a chicken hen is made up of two parts: the ovary and the oviduct. Ova (yolks) develop in the ovary. When an ovum matures, it is released from the ovary into the oviduct. This release of the ovum is ovulation. In the oviduct, glands secrete substances that form other parts of the egg, such as the albumen (egg white) and the shell.
The male chicken contributes to reproduction by producing sperm in the testes, which are carried in the semen. The male reproductive system consists of two testes, each with a deferent duct that leads to the cloaca. During reproduction, the rooster deposits semen into the hen's cloaca in a process called a cloacal kiss.
The hen's reproductive system contains sperm storage tubules (SSTs), also known as sperm nests, which are located in the oviduct. These SSTs are pouches or nests in the reproductive tract where previously deposited sperm can be stored for later fertilization of eggs. The number of SSTs a hen has is determined by her genetics, and hens with more SSTs can store sperm for longer. The longevity of stored sperm is limited, with a mating session only fertilizing eggs for 4-5 days. However, stored sperm can fertilize multiple eggs over time, and hens can store sperm from multiple males.
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The longevity of the sperm is limited
The reproductive system of a female chicken is made up of two parts: the ovary and the oviduct. Ova (yolks) develop in the ovary, and when an ovum matures, it is released from the ovary into the oviduct. This release of the ovum is ovulation. In the oviduct, glands secrete substances that form other parts of the egg, such as the albumen (egg white) and the shell. The total time a hen’s body takes to transform a yolk into a fully developed egg and lay that egg is about 25 to 26 hours.
The male chicken, or rooster, has two testes and two vasa deferentia (the duct through which sperm are transported from the testes). The vas deferens is also the main area of sperm storage in male chickens. Applying external pressure in this area results in ejaculation, and the collection of sperm in this way for artificial insemination of hens is often referred to as "milking the rooster". Each vas deferens opens into a small bump, or papilla, on the back wall of the cloaca.
The longevity of chicken sperm is limited. Rooster semen can fertilize many eggs, but one mating session only has the potential to fertilize eggs for 4-5 days. There is no way to tell if an egg is fertile when it is laid. The only way to determine if fertilization has occurred is to "candle" the egg after at least 4 days of incubation. The egg is fertilized about 24 hours before it is laid, and sperm of any species does not survive more than 24 to 48 hours.
Flock fertility is dependent on the reproductive status of the chickens (the level of egg and semen production) combined with the chickens’ interest in and capability of mating. Fertility of both male and female chickens tends to decrease as the chickens get older. For females, it is believed that the decline in fertility is due to the faster release of sperm from the sperm storage tubules within the chicken’s reproductive system, meaning that the hen cannot store sperm as long and, therefore, requires more frequent mating. For males, it is presumed that although roosters continue to produce sperm for many years, sperm quality declines and mating activity decreases as a rooster ages.
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Sperm is stored in special nests in the oviduct
The reproductive system of a chicken hen is made up of two parts: the ovary and the oviduct. The ovary is a cluster of developing ova, located midway between the neck and the tail of the bird and attached at the back. In almost all species of birds, only the left ovary and oviduct are functional.
The longevity of the sperm is limited, and one mating only has the potential to fertilize eggs for 4-5 days. However, hens can store viable sperm for around 21 days. The number of SSTs a hen has is inherited through her genetics, and hens with more SSTs can store sperm for longer than hens with fewer SSTs. This means that the fertility of the hen is increased, as the hen does not need to mate again to fertilize additional eggs.
The rooster deposits enough semen to fertilize many eggs. Once sperm is deposited in the hen's cloaca, it travels through a series of barriers that act as quality control checkpoints to weed out substandard sperm. Very few sperm get past the next hurdle and on to the SSTs.
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Sperm storage tubules are also called pouches or nests
In female chickens, the reproductive system is made up of an ovary and an oviduct. The ovary is a cluster of developing ova, which can develop into yolks. The oviduct is where the yolk is fertilised and the albumen, shell membranes and shell are added. The oviduct is also where the sperm is stored in 'sperm storage tubules', also called 'pouches' or 'nests'. These tubules are cylindrical tubes hidden in the folds of vaginal tissue at two sites: in the lower portion of the infundibulum or the more commonly used ones found in the upper portion of the vagina at the utero-vaginal junction (UVJ).
The number of sperm storage tubules (SSTs) a hen has is inherited through her genetics. The more SSTs a hen has, the more sperm she can store, and the longer she can store it for. Hens with more SSTs therefore have higher fertility rates. The longevity of the sperm is limited, and one mating only has the potential to fertilise eggs for 4-5 days. However, it is believed that hens can store viable sperm for around 21 days.
The process by which hens store sperm is as follows: first, the rooster deposits semen in the hen's cloaca, which is referred to as a 'cloacal kiss'. The sperm then travels through a series of barriers that act as quality control check-points, rejecting dead, immobile, or poor 'swimmer' sperm. Very few sperm get past this first barrier and on to the SSTs. If high amounts of fatty acids are released into the SSTs, they may damage the sperm and impair the hen's ability to store it. However, some hens have a gene that allows them to break down these fatty acids, improving the sperm's survival.
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Sperm is deposited in the hen's cloaca
The reproductive system of a chicken hen is made up of two parts: the ovary and the oviduct. The ovary is a cluster of developing ova (yolks), located midway between the neck and the tail of the bird and attached at the back. The oviduct is where the yolk is fertilised and has added to it the albumen, shell membranes and shell.
The rooster deposits semen in the hen's cloaca, which is known as a "cloacal kiss". The cloaca is a chamber located at the end of the digestive tract, and it is the only opening for the urinary, reproductive, and intestinal tracts in birds. The semen contains millions of sperm, which then race through a series of barriers that act as quality control checkpoints to weed out substandard sperm.
The sperm that make it through the barriers continue on to the sperm storage tubules (SSTs) or "sperm nests", which are located in the oviduct. The SSTs are pouches or nests in the hen's reproductive tract where previously deposited sperm can be stored for a period of time, typically 10 days to 2 weeks, though some sources suggest up to 21 days. This allows the hen to maximise fertilisation as stored sperm can fertilise multiple eggs over time, meaning she doesn't need to mate again to fertilise additional eggs.
The number of SSTs a hen has is determined by her genetics, and hens with more SSTs can store sperm for longer. The successful long-term storage of sperm requires ideal conditions in the reproductive tract. Hens that are able to break down fatty acid secretions in the reproductive tract have higher fertility rates, as high amounts of fatty acids can cause irreversible damage to sperm.
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Frequently asked questions
The sperm nest, also known as sperm storage tubules, are located in the oviduct of hens.
The sperm nest collects and stores semen for later fertilization of eggs. This allows hens to maximize fertilization without needing to mate again to fertilize additional eggs.
Sperm can be stored in the sperm nest for up to 21 days, with high fertility for about 1 week, some fertility for 2 weeks, and low fertility for 3 weeks.









































